One valuable application of immunochemistry is the characterization of neoplastic cells on the basis of specific surface, cytoplasmic, and/or nuclear protein expression.

16 7455 Canine malignant melanoma 50x WEB16 7455 Canine malignant melanoma ICC melanA positive 50x WEB

[Dog. Cytologic preparation. Malignant melanoma. LEFT: Wright-Giemsa. RIGHT: Melan-A, 50X objective]

Immunocytochemistry (on cytologic preparations) or immunohistochemistry (on formalin-fixed paraffin-embedded tissues) may be recommended in tumors where cellular differentiation is insufficient to allow definitive diagnosis, and differential diagnoses include neoplasms with significantly different biological behaviors. Immunochemistry is an ancillary diagnostic technique and must always be interpreted in conjunction with morphologic features of neoplastic cells as well as in conjunction with the overall clinical picture. Frequently, a panel of antibodies is recommended for the interrogation of a particular neoplasm. Below is a list of optimized immunochemical markers available at SpecialtyVETPATH.  We continue to expand this list so please don't hesitate to inquire about or request additional markers.

  • Cytokeratin
  • Vimentin
  • Smooth muscle actin
  • Myosin
  • CD20
  • CD18
  • CD79a
  • CD3
  • CD117 (c-kit)
  • Melan-A
  • Mum1